Translation initiation factor eIF-2. Cloning and expression of the human cDNA encoding the gamma-subunit.
作者： N.J. Gaspar , T.G. Kinzy , B.J. Scherer , M. Hümbelin , J.W. Hershey
DOI: 10.1016/S0021-9258(17)41878-3
关键词: Molecular biology 、 Protein subunit 、 Peptide sequence 、 Complementary DNA 、 Molecular cloning 、 Biology 、 Eukaryotic Initiation Factor-2 、 cDNA library 、 Gamma subunit 、 Protein primary structure
摘要: Translation initiation factor eIF-2 is a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA, to 40 S ribosomal subunit. To complete our characterization eIF-2, we cloned and characterized human cDNA encoding largest subunit, gamma. From limited peptide sequence data, degenerate oligo-nucleotide primers were designed amplify 118-base pair DNA fragment from library. This was used as probe screen for larger cDNAs eventually clone containing gamma coding region (1416 base pairs) identified. It encodes 472-amino acid (51.8 kDa) contains three consensus elements. The shares strong homology EF-Tu, GCD11 (the yeast homolog gamma), other EF-Tu-like proteins. Transfection COS-1 cells with results overexpression 52-kDa which specifically recognized by anti-eIF-2 antibodies. Cross-linking experiments diepoxybutane trans-diaminedichloroplatinum(II) indicate that both beta- gamma-subunits are close proximity methionyl-tRNAi ternary complexes. Possession will facilitate future investigations interactions GTP eIF-2.
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