IGFBP5 enhances osteogenic differentiation potential of periodontal ligament stem cells and Wharton's jelly umbilical cord stem cells, via the JNK and MEK/Erk signalling pathways

摘要: Objectives Mesenchymal stem cell (MSC)-mediated tissue regeneration represents a promising strategy for repair of defects, but its molecular mechanisms remain unclear, restricting the use MSCs. Our previous study indicated that insulin-like growth factor-binding protein 5 (IGFBP5) exerted valuable effect on osteogenic differentiation MSCs, underlying directed remained unclear. In this study, we have investigated role IGFBP5 in regulating potential. Materials and methods Periodontal ligament cells (PDLSCs) were isolated from periodontal tissue. Wharton's jelly umbilical cord (WJCMSCs) was obtained commercially. Lentiviral shRNA used to silence IGFBP5. Retroviruses expressing wild-type overexpress WJCMSCs. Recombinant human (rhIGFBP5) treat PDLSCs 24 h. Western blot analysis detect MAPK signalling pathway, alkaline phosphatase (ALP) activity, Alizarin Red staining quantitative calcium potentials. Results Overexpression or rhIGFBP5 increased expression levels phosphorylated c-Jun N-terminal kinase (p-JNK), mitogen-activated 1 2 (p-MEK1/2) extracellular regulated kinases (p-Erk1/2) both WJCMSCs PDLSCs. Consistently, silenced found effectively inhibit p-JNK, p-Erk1/2 p-MEK1/2 Furthermore, inhibition JNK by inhibitor, SP600125, MEK/Erk PD98059, dramatically blocked IGFBP5-enhanced ALP activity vitro mineralization WJCMSCs. Conclusions Our results demonstrated promoted potentials via pathways.