Characterization of cloned cDNA representing rat myelin basic protein: Absence of expression in brain of shiverer mutant mice
作者： Arthur Roach , Kevin Boylan , Suzanna Horvath , Stanley B. Prusiner , Leroy E. Hood
DOI: 10.1016/0092-8674(83)90536-6
关键词: Mutant 、 Wild type 、 Gene 、 Messenger RNA 、 Biology 、 cDNA library 、 Molecular biology 、 Myelin basic protein 、 Peptide sequence 、 Biochemistry 、 Complementary DNA 、 General Biochemistry, Genetics and Molecular Biology
摘要: A cDNA library was constructed from mRNA isolated the brains of 18-day-old rats, age at which myelin biosynthesis is maximal. synthetic DNA probe synthesized based on reverse translation amino acid sequence rat basic protein (MBP) used to select two clones encoding MBP. 1.5 kb Eco RI fragment one clone completely sequenced. When translated, a portion this identical 126 127 positions with reported for small MBP rat. Brains mice homozygous shiverer genotype contained neatly reduced amounts relative wild type. deletion sequences in genome also demonstrated. cDNAs will allow molecular investigation role gene plays both dysmyelinating and demyelinating diseases, as well questions biosynthesis.
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