Monoclonal Antibody to Human Midkine Reveals Increased Midkine Expression in Human Brain Tumors

摘要: We produced a rat IgG2a monoclonal antibody against the carboxyl terminal region of human midkine (MK), novel growth factor. This was used in immunohistochemical studies to compare expression MK, proliferating cell nuclear antigen (PCNA) and p53 protein 133 primary brain tumors 21 carcinoma metastases central nervous system. Approximately half glioblastomas multiforme (GBMs) (19/32), medulloblastomas (8/14), primitive neuroectodermal (PNETs) (5/11), breast (Br-Mts) (6/10) lung (L-Mts) (5/11) as well some astrocytomas (2/14) had tumor cells that expressed MK; however, oligodendrogliomas, ependymomas, schwannomas, meningiomas, pituitary adenomas did not express MK. The values PCNA-labeling index were statistically higher GBMs, medulloblastomas, PNETs, Br-Mts, L-Mts MK than those (Wilcoxon rank-sum test, p < 0.05). There no correlation between all types. Normal non-neoplastic tissues negative for PCNA, protein. conclude metastatic may depend, part, on potential.