作者: S NOBATA , T SHINOZAWA , A SAKANISHI
DOI: 10.1016/J.CANLET.2004.07.003
关键词: Transformation (genetics) 、 Midkine 、 Reverse transcription polymerase chain reaction 、 Molecular biology 、 In vivo 、 Fetal bovine serum 、 Real-time polymerase chain reaction 、 Carcinogenesis 、 Biology 、 Transfection 、 Cancer research 、 Oncology
摘要: To test whether truncated midkine (tMK), an alternative spliced form of exon 3, induces the transformation mammalian cells, tMK cDNA was transfected into SW-13 cells. Although, growth MK transfectant (SW-13/MK) cells close to those parent and vector (SW-13/empty) (SW-13/tMK) significantly promoted compared with that above three Both SW-13/tMK SW-13/MK formed colonies in 0.35% soft agar, indicating induce cell transformation. frequently solid tumor after its subcutaneous injection nude mice. Additionally, SW-13/MK-injected mice, advanced visible tumors were detected case SW-13/empty-injected mice as control. These findings indicate promotes establishment vivo.