Automated behavioral analysis of limbs’ activity in the forced swim test

摘要: The forced swim test in rodents has been widely used to evaluate potential effectiveness of antidepressant medications since it was described 1977 by Porsolt. In this test, a rodent is placed water container, and its immobility time measured. indicates the level inactivity, interpreted as 'hopelessness', shown decrease when treated with medications. simple measure does not take into account intermediate behaviors during testing (ranging from total extensive 'struggling' behavior) show normal Gaussian distribution tested groups rats. We have previously developed software allowing an observer assign scores full range continuously reporting motion limbs using joystick. Based on joystick score, we now automatic tool that uses computer vision algorithm (CVA) analyze specifically generate objective, reproducible automated score. current study analyzed data obtained traditional time, analysis new CVA method group 20 addition, effects various these different scoring methods. validated positively correlated Data reproducible, significantly reduces required for human analysis.