Controlling mesopore size and processability of transparent enzyme-loaded silica films for biosensing applications.

摘要: Silica-based nanoporous thin films including large mesopores are relevant as enzyme supports for applications in biosensing. The diffusion and immobilization of biomolecules such enzymes porous require the presence mesopores. Creating morphologies based on a bottom-up synthesis using colloidal templates is challenge view combination desired material properties robustness casting process fabrication films. Here strategy to reproducibly synthesize transparent silica with submicrometer thickness homogeneously distributed porosity presented. For this purpose, polystyrene-poly-2-vinylpyridine (PS-P2VP) amphiphilic block copolymers used porogenic templates. Low-chain alcohols employed both selective solvents P2VP blocks reaction media synthesis. Rheology measurements reveal strong influence copolymer length behavior PS-P2VP micelles suspen...