Rapid in planta detection of Chalara fraxinea by a real-time PCR assay using a dual-labelled probe

作者: Renaud Ioos , Tadeusz Kowalski , Claude Husson , Ottmar Holdenrieder

DOI: 10.1007/S10658-009-9471-X

关键词: Agar plateHymenoscyphus fraxineusPolymerase chain reactionIsolation (microbiology)PathogenMicrobiologyAgarIn silicoInternal transcribed spacerBiology

摘要: … a dual-labelled probe to be used in a real-time PCR assay for the detection of C. fraxinea. The test proved to be specific, based on in silico and in vitro assessments, and could detect as …

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