Direct Comparison of the Insulating Properties of Two Genetic Elements in an Adenoviral Vector Containing Two Different Expression Cassettes
作者： Pilar Martin-Duque , Sarah Jezzard , Lambrini Kaftansis , Georges Vassaux
关键词: Thymidine kinase 、 Transcription (biology) 、 Viral vector 、 Gene expression 、 Biology 、 Promoter 、 Chromatin 、 Gene 、 Molecular biology 、 Expression cassette 、 Molecular medicine 、 Genetics
摘要: Targeted gene expression can be achieved through the use of cell-selective promoters. However, when cassette is delivered by an adenovirus, "promoter interference," resulting in loss specificity, has been reported. To overcome this problem, insulator elements (the bovine growth hormone transcription stop signal or HS4 chromatin insulators chicken β-globin locus) have used. The present study examines efficacy these elements, two independent cassettes (one which a strong, ubiquitous promoter drives green fluorescent protein and another "cancer-selective" ERBB2 herpes simplex virus thymidine kinase [HSVtk] gene) are incorporated into same recombinant adenovirus. As expected, presence either does not alter HSVtk ERBB2-positive cells, measured sensitization cells to ganciclovir. When ERBB2-negative were inf...
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