Differential sensitivity of head and neck cancers to non-major histocompatibility-restricted killer cell activity.

摘要: Cell lines derived from squamous cell carcinoma of the upper aerodigestive tract (head and neck cancer) were phenotypically characterized with regard to differential sensitivity nonmajor histocompatibility restricted (non-MHCr) killer activity. Requirements for detectable lysis in a standard chromium release assay included either isolation fresh enriched Leu 19+ large granular lymphocytes (both 19+CD3+ 19+CD3- populations) or interleukin-2 (IL-2) stimulation peripheral blood (PBL). In neither circumstance could lytic activity be identified among 19- populations. With PBL IL-2 significant expressed by head cancer (P < 0.001 analysis variance) was maintained regardless source, i.e., healthy controls three differing populations patients categorized disease status treatment. One factor associated line's increased degree tumor differentiation, poorly differentiated tumors (as defined intermediate filament cytochemical staining [decreased keratin vimentin]) being more sensitive. Furthermore, as increased, major complex (MHC)-class I antigen expression diminished concurrently. 1 4 tested, however, pretreatment cells interferon-γ induced independent changes MHC-class expression, indicating factors not related are likewise relevant. previous studies we vivo prognostic significance non-MHCr cytotoxicity against K562 targets, principally predictive metastatic development persons cancers. This report extends these observations demonstrating vitro that target highly sensitive function effector cells.