A multicellular tumor spheroid model of cellular immunity against head and neck cancer.

摘要: A multicellular tumor spheroid (MTS) model for head and neck cancers has been used to examine the immune function of fresh 6-day interleukin-2(IL-2)-activated peripheral blood lymphocytes (PBL). MTS are individually cultured in presence effector cells, spheroids' growth is monitored by sizing them under an inverted microscope. Dose/response studies IL-2 (0–100 U/ml) alone unstimulated PBL (0–105 cells/MTS) showed no effects on growth. IL-2-activated cells/MTS), contrast, modulated a multiphasic pattern: was unperturbed first 3 days then inhibition occurred, followed disintegration. Histological analysis that intact histoarchitecture correlated with growth, increasing cell sloughing dissolution replacement activated Flow-cytometric sorting lymphocyte subset populations indicated it Leu19+CD3− cells produced these growth-modulatory effects. In contrast initial LAK resistance MTS, single-cell suspensions demonstrated significant lysis standard 4-h chromium-release assays. Differences between single suggest potential tissue-like organization as factor lymphokine-activated killing.