Phencyclidine treatment in mice: Effects on phencyclidine binding sites and glutamate uptake in cerebral cortex preparations

摘要: The effects of a psychotomimetic drug, phencyclidine (PCP), on glutamatergic neurotransmission were studied in mice. binding tritiated N-[1-(2-thienyl)cyclohexyl]piperidine (TCP) to cerebral cortical membranes and the uptake [3H]glutamate by synaptosomal preparations assessed after PCP treatment (1 mg/d/mouse for 3 days) with implanted minipumps. capacity Bmax TCP significantly increased but constant KD remained same exposure, indicating that more sites became available. basic properties unaltered actions glutamate, glutamate receptor agonists glycine potentiated PCP-treated was saturable, consisting both high- low-affinity transport components. After exposure Km high-affinity component not changed. maximal velocity V while decreased. Moreover, inhibition structural analogues potentiated, suggesting modification transporter. results show chronic treatment, used as model psychosis, markedly affects parameters.