Phospholipase A2-catalyzed hydrolysis of lecithin in a continuous reversed-micellar membrane bioreactor
作者： M.A.P. Morgado , J.M.S. Cabral , D.M.F. Prazeres
DOI: 10.1007/BF02523428
关键词: Enzymatic hydrolysis 、 Phospholipid 、 Bioreactor 、 Chromatography 、 Lecithin 、 Membrane bioreactor 、 Phosphatidylcholine 、 Chemistry 、 Membrane reactor 、 Membrane
摘要: Lysophospholipids and free fatty acids produced by lecithin hydrolysis are important natural compounds with high potential for application in the food, chemical, and pharmaceutical industries. In this work, the enzymatic hydrolysis of lecithin (essentially phosphatidylcholine) catalyzed by porcine pancreatic phospholipase A2 (phosphatide 2‐acyl‐hydrolase, EC 3.1. 1.4), encapsulated in mixed reversed micelles of lecithin andbis (2‐ethylhexyl) sodium sulfosuccinate (AOT) in isooctane, was carried out in a continuous reversed‐micellar …
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sci-hub.se 参考文章(20)
J. A. L. Santos, M. Mateus, J. M. S. Cabral, Pressure Driven Membrane Processes Chromatographic and Membrane Processes in Biotechnology. pp. 177- 205 ,(1991) , 10.1007/978-94-011-3470-5_9
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
M. A. P. Morgado, J. M. S. Cabral, D. M. F. Prazeres, Hydrolysis of lecithin by phospholipase A2 in mixed reversed micelles of lecithin and sodium dioctyl sulphosuccinate Journal of Chemical Technology & Biotechnology. ,vol. 63, pp. 181- 189 ,(1995) , 10.1002/JCTB.280630214
D.M.F. Prazeres, J.M.S. Cabral, Enzymatic membrane bioreactors and their applications Enzyme and Microbial Technology. ,vol. 16, pp. 738- 750 ,(1994) , 10.1016/0141-0229(94)90030-2
D. M. F. Prazeres, F. A. P. Garcia, J. M. S. Cabral, Kinetics and stability of a Chromobacterium viscosum lipase in reversed micellar and aqueous media. Journal of Chemical Technology & Biotechnology. ,vol. 53, pp. 159- 164 ,(2007) , 10.1002/JCTB.280530209
Karel Martinek, N.L. Klyachko, A.V. Kabanov, Yu.L. Khmelnitsky, A.V. Levashov, Micellar enzymology: its relation to membranology Biochimica et Biophysica Acta. ,vol. 981, pp. 161- 172 ,(1989) , 10.1016/0005-2736(89)90024-2
Douglas B. Sarney, Giuseppe Fregapane, Evgeny N. Vulfson, Lipase-catalyzed synthesis of lysophospholipids in a continuous bioreactor Journal of the American Oil Chemists' Society. ,vol. 71, pp. 93- 96 ,(1994) , 10.1007/BF02541478
Tae-Jong Kim, Yun-Dong Lee, Hak-Sung Kim, Enzymatic production of cyclodextrins from milled corn starch in an ultrafiltration membrane bioreactor. Biotechnology and Bioengineering. ,vol. 41, pp. 88- 94 ,(1993) , 10.1002/BIT.260410112
John W. Shield, Holly D. Ferguson, Andreas S. Bommarius, T. Alan Hatton, Enzymes in reversed micelles as catalysts for organic-phase synthesis reactions Industrial & Engineering Chemistry Fundamentals. ,vol. 25, pp. 603- 612 ,(1986) , 10.1021/I100024A022
H. Strathmann, Membrane separation processes Journal of Membrane Science. ,vol. 9, pp. 121- 189 ,(1981) , 10.1016/S0376-7388(00)85121-2