Expression and purification of amyloid β-protein, tau, and α-synuclein in Escherichia coli: a review.
作者： Longgang Jia , Wenping Zhao , Wei Wei , Xiao Guo , Wenjuan Wang
DOI: 10.1080/07388551.2020.1742646
关键词: Tau protein 、 α synuclein 、 Amyloid β 、 Purification methods 、 Neurotoxicity 、 Biochemistry 、 Escherichia coli 、 Recombinant DNA 、 Amyloid 、 Chemistry
摘要: Misfolding and accumulation of amyloidogenic proteins into various forms aggregated intermediates insoluble amyloid fibrils is associated with more than 50 human diseases. Large amounts high-quality are required for better probing their aggregation neurotoxicity. Due to intrinsic hydrophobicity, it a challenge obtain high yield purity, they have attracted the attention researchers from all over world. The rapid development bioengineering technology provides technical support obtaining large recombinant proteins. This review discusses available expression purification methods three including β-protein, tau, α-synuclein in microbial systems, especially Escherichia coli, advantages disadvantages these methods. Importantly, protocols can also be referred other hydrophobic
sci-hub.se 参考文章(107)
Zhong-Ying Mo, Ying-Zhu Zhu, Hai-Li Zhu, Jun-Bao Fan, Jie Chen, Yi Liang, Low Micromolar Zinc Accelerates the Fibrillization of Human Tau via Bridging of Cys-291 and Cys-322 Journal of Biological Chemistry. ,vol. 284, pp. 34648- 34657 ,(2009) , 10.1074/JBC.M109.058883
Bertil Macao, Wolfgang Hoyer, Anders Sandberg, Ann-Christin Brorsson, Christopher M Dobson, Torleif Härd, Recombinant amyloid beta-peptide production by coexpression with an affibody ligand. BMC Biotechnology. ,vol. 8, pp. 82- 82 ,(2008) , 10.1186/1472-6750-8-82
Joseph T. Jarrett, Elizabeth P. Berger, Peter T. Lansbury, The carboxy terminus of the beta amyloid protein is critical for the seeding of amyloid formation: implications for the pathogenesis of Alzheimer's disease. Biochemistry. ,vol. 32, pp. 4693- 4697 ,(1993) , 10.1021/BI00069A001
Tristan Bolmont, Florence Clavaguera, Melanie Meyer-Luehmann, Martin C. Herzig, Rebecca Radde, Matthias Staufenbiel, Jada Lewis, Mike Hutton, Markus Tolnay, Mathias Jucker, Induction of Tau Pathology by Intracerebral Infusion of Amyloid-β-Containing Brain Extract and by Amyloid-β Deposition in APP × Tau Transgenic Mice American Journal of Pathology. ,vol. 171, pp. 2012- 2020 ,(2007) , 10.2353/AJPATH.2007.070403
Adriano Aguzzi, Tracy O'Connor, Protein aggregation diseases: pathogenicity and therapeutic perspectives Nature Reviews Drug Discovery. ,vol. 9, pp. 237- 248 ,(2010) , 10.1038/NRD3050
J. W. Chin, A. B. Martin, D. S. King, L. Wang, P. G. Schultz, Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 11020- 11024 ,(2002) , 10.1073/PNAS.172226299
Makkapati Satakarni, Robin Curtis, Production of recombinant peptides as fusions with SUMO Protein Expression and Purification. ,vol. 78, pp. 113- 119 ,(2011) , 10.1016/J.PEP.2011.04.015
J. A. Driver, G. Logroscino, J. M. Gaziano, T. Kurth, Incidence and remaining lifetime risk of Parkinson disease in advanced age. Neurology. ,vol. 72, pp. 432- 438 ,(2009) , 10.1212/01.WNL.0000341769.50075.BB
Masami Masuda, Naoshi Dohmae, Takashi Nonaka, Takayuki Oikawa, Shin-ichi Hisanaga, Michel Goedert, Masato Hasegawa, Cysteine misincorporation in bacterially expressed human α-synuclein FEBS Letters. ,vol. 580, pp. 1775- 1779 ,(2006) , 10.1016/J.FEBSLET.2006.02.032
Abhishek Chatterjee, Sophie B. Sun, Jennifer L. Furman, Han Xiao, Peter G. Schultz, A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli Biochemistry. ,vol. 52, pp. 1828- 1837 ,(2013) , 10.1021/BI4000244