Interaction between pyridoxal kinase and pyridoxal-5-phosphate-dependent enzymes.
作者： Pik-Yuen Cheung , Chi-Chun Fong , Kang-To Ng , Wan-Chuen Lam , Yun-Chung Leung
DOI: 10.1093/JB/MVG201
关键词: Surface plasmon resonance 、 Binding affinities 、 Pyridoxal kinase 、 Enzyme 、 Pyridoxal 5-Phosphate 、 Biochemistry 、 Glutamate decarboxylase 、 Fluorescence anisotropy 、 Stereochemistry 、 Alanine aminotransferase 、 Chemistry
摘要: The interactions of two pyridoxal-5-phosphate (PLP)-dependent enzymes, alanine aminotransferase (ALT) and glutamate decarboxylase (GAD), with pyridoxal kinase (PK) were studied by fluorescence polarization as well surface plasmon resonance techniques. results demonstrated that PK can specifically bind to ALT GAD. Moreover, binding profiles both enzymes immobilized altered excess amount PLP. equilibrium affinity constants for in the absence presence PLP are 20.4 x 10 4 M - 1 6.7 , GAD 37 20.8 respectively. It appears specific occur between PLP-dependent affinities decrease support our hypothesis transfer from requires a interaction enzyme.
core.ac.uk
oup.com
oupjournals.org参考文章(25)
Burton M. Pogell, ENZYMATIC OXIDATION OF PYRIDOXAMINE PHOSPHATE TO PYRIDOXAL PHOSPHATE IN RABBIT LIVER Journal of Biological Chemistry. ,vol. 232, pp. 761- 776 ,(1958) , 10.1016/S0021-9258(19)77396-7
Pascale Lainé-Cessac, Pierre Allain, Kinetic studies of the effects of K+, Na+ and Li+ on the catalytic activity of human erythrocyte pyridoxal kinase. Enzyme and Protein. ,vol. 49, pp. 291- 304 ,(1996) , 10.1159/000468639
Y. C. Leung, H. Y. Wong, J. E. Churchich, S. C. L. Lo, F. Kwok, Structure-function relationships of porcine pyridoxal kinase Birkhäuser Verlag. pp. 277- 280 ,(2000) , 10.1007/978-3-0348-8397-9_45
Y T Kim, F Kwok, J E Churchich, Interactions of pyridoxal kinase and aspartate aminotransferase emission anisotropy and compartmentation studies. Journal of Biological Chemistry. ,vol. 263, pp. 13712- 13717 ,(1988) , 10.1016/S0021-9258(18)68299-7
G DeRosa, RW Swick, Metabolic implications of the distribution of the alanine aminotransferase isoenzymes. Journal of Biological Chemistry. ,vol. 250, pp. 7961- 7967 ,(1975) , 10.1016/S0021-9258(19)40801-6
Jang-Yen Wu, Larry Denner, Chin-Tarng Lin, Guoxing Song, L-Glutamate decarboxylase from brain. Methods in Enzymology. ,vol. 113, pp. 3- 10 ,(1985) , 10.1016/S0076-6879(85)13004-1
Haoyuan Chen, Donald B. McCormick, Update on Interconversions of Vitamin B-6 with Its Coenzyme Journal of Nutrition. ,vol. 129, pp. 325- 327 ,(1999) , 10.1093/JN/129.2.325
Man-Sau Wong, Chi-Chun Fong, Mengsu Yang, Biosensor measurement of the interaction kinetics between insulin-like growth factors and their binding proteins. Biochimica et Biophysica Acta. ,vol. 1432, pp. 293- 301 ,(1999) , 10.1016/S0167-4838(99)00106-5
Ting-Kai Li, Lawrence Lumeng, Robert L. Veitch, Regulation of pyridoxal 5'-phosphate metabolism in liver. Biochemical and Biophysical Research Communications. ,vol. 61, pp. 677- 684 ,(1974) , 10.1016/0006-291X(74)91010-9
Chi-Chun Fong, Wan-Ping Lai, Yun-Chung Leung, Samuel C.-L. Lo, Man-Sau Wong, Mengsu Yang, Study of substrate-enzyme interaction between immobilized pyridoxamine and recombinant porcine pyridoxal kinase using surface plasmon resonance biosensor. Biochimica et Biophysica Acta. ,vol. 1596, pp. 95- 107 ,(2002) , 10.1016/S0167-4838(02)00208-X