Synchronization of mammalian cells in vitro by inhibition of the DNA synthesis. II. Population dynamics.

摘要: Summary Some aspects of the population dynamics before, during and after double thymidine treatment synchronization procedure were analysed. The mitotic index in synchronized populations rose from 0.0 per cent at 1 hr end second to 20.8 9.7 hr, dropped 0.1 about 22 increased again a peak 8 31 hr. percentage mitoses controls was 2.1. degree first generation, determined according Engelberg, 73 cent. S phase cells fell 96 0–3.5 via 50 6.5 12–15 72 24 decreased 7 34 mean length G 2 phases cycle shorter than asynchronous controls, while duration mitosis equal both cases. other characteristics generation are discussed. It argued that this system probably consequence reversible alteration life cells, induced by thymidine. greatly presence biochemical implications concerning blocking deblocking DNA synthesis administration removal