Correlation between synthesis of heat shock proteins and development of thermotolerance in Chinese hamster fibroblasts.

摘要: Synthesis of a family proteins called "heat shock" is induced or enhanced in cells response to various environmental stresses, suggesting that these may perform functions essential cell survival. Because brief, nonlethal heat treatment can dramatically induce transient resistance subsequent lethal (thermotolerance), we examined the effect (41-46 degrees C) on protein synthesis and survival plateau-phase Chinese hamster fibroblast (HA-1) cells. After treatments either drastically inhibited total (46 did not suppress it (41 C), shock was greatly over unheated cells, increased 10(2)- 10(6)-fold when were challenged by treatment. The correlated well with development thermotolerance, stability persistence thermotolerance up 36 hr. Sodium arsenite, hypoxia, ethanol also both thermotolerance. A qualitative analysis individual suggests polypeptides Mr 70,000 87,000 most closely conformed kinetics