Evaluation of the Abbott Real-Time HIV-1 quantitative assay with dried blood spot specimens

作者: A. Marconi , M. Balestrieri , G. Comastri , F.R. Pulvirenti , W. Gennari

DOI: 10.1111/J.1469-0691.2008.02116.X

关键词: Viral loadQuantitative analysis (chemistry)VirusRNADried blood spotSample preparationMedicineVirologyChromatographyHuman immunodeficiency virus (HIV)Quantitative assay

摘要: Abstract The Abbott Real-Time HIV-1 assay was evaluated for its performance in quantification of human immunodeficiency virus type 1 (HIV-1) RNA dried blood spot (DBS) samples. In total, 169 samples with detectable plasma were used to extract from paired DBS and liquid samples, using the automated m Sample Preparation System (m2000sp). then quantitated by m2000rt RealTime analyser. suitable real-time PCR obtained all but one (99.4%) detected 163/168 (97.0%) correlation between values measured very high ( r = 0.882), 78.5% 99.4% cases differing

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