Differential density labeling and gradient centrifugation of Tetrahymena: a new procedure for selection synchrony.
作者： J. Wolfe
DOI: 10.1016/0014-4827(73)90572-7
关键词: Population 、 Biophysics 、 Botany 、 Biology 、 Generation time 、 Division (mathematics) 、 Tetrahymena 、 Centrifugation 、 Cell cycle 、 Interphase 、 Ficoll
摘要: Abstract A simple technique has been developed which selectively separates late division and post-division cells from a culture of logarithmically growing, asynchronous Tetrahymena . The takes advantage the division-related cessation feeding. After 5 min exposure to minute particles tantalum, 90% population will have incorporated concentrated into food vacuoles. remaining 10% constitutes population. Centrifugation through Ficoll step-gradient non-feeding therefore less heavy feeding, interphase separated show peak at 170 ± 10 cell number is doubled by division. bulk DNA synthesis, as measured 3 H-thymidine incorporation occurs between 30 120 min. At least with respect generation time duration replication selection procedure leaves cycle unaltered.
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