Analysis of picogram quantities of indole-3-acetic acid by high performance liquid chromatography-fluorescence procedures.

作者: Alan Crozier , Karen Loferski , Joe B. Zaerr , Roy O. Morris

DOI: 10.1007/BF00390171

关键词: High-performance liquid chromatographyIndole-3-acetic acidAnalytical chemistryChemistryFluorescenceChromatography

摘要: The use of spectrofluorimeter coupled to a reverse phase high performance liquid chromatography column permits selective detection indole-3-acetic acid at the low picogram level. value technique is demonstrated by analysis endogenous IAA in elongating shoots, xylem sap and callus Douglas-fir. data are also used illustrate procedure whereby accuracy chromatographic analyses can be verified within definable probability limits.

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