High Performance Liquid Chromatography and the Analysis of Indole-3-Acetic Acid, and Some of Its Decarboxylated Catabolites in Scots Pine (Pinus sylvestris L.)

摘要: Indole-3-acetic acid (1) (IAA) is a weak organic with pKa of 4.8 and an important growth-regulating substance in higher plants (see Letham et al. 1978). The biological activity submicrogram amounts IAA has been demonstrated by exogenous applications to several different species tissues. It believed that endogenous involved the regulation plant growth development, controlling processes such as cell elongation differentiation. first conclusive evidence presence plants, was obtained Zea mays Haagen-Smit (1946). Since then identified constituent number its function regulator thought be universal. concentration tissues usually around 1–100 ng g−1 fresh weight. When investigating hormonal role IAA, it common practice attempt correlate changes levels developmental event under study. However, this approach will limited value if amount available for related rate turnover rather than pool size. therefore essential investigate metabolism pathways monitor not only pools but also those precursors catabolites.