Molecular species identification of scat samples of South American felids and canids

摘要: Carnivores play an important role on the ecosystem equilibrium. However, due to habitat loss and fragmentation, their populations have been declining around world. Together, elusive behavior often low population density challenge data collection. In this way, non-invasive sampling has considered as alternative for information gathering without need of animal capture. Molecular analysis essential precise species identification through samples, scats. DNA from these samples is usually found degraded, use more than one marker a safer critical. work, we tested set new mini barcoding primers molecular Neotropical felid canid species. Primers three mitochondrial regions—rRNA12Ss1, rRNA12Ss2, rRNA16S—were designed amplified using fecal while control region CytB were only canids, totaling five primer pairs successfully in samples. This study resulted very efficient set, which represents tool South American carnivores based