Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP+)-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway

摘要: Neuroinflammation is closely associated with the pathophysiology of neurodegenerative diseases including Parkinson's disease (PD). Recent evidence indicates that astrocytes also play pro-inflammatory roles in central nervous system (CNS) by activation toll-like receptor (TLR) ligands. Therefore, targeting anti-inflammation may provide a promising therapeutic strategy for PD. Curcumin, polyphenolic compound isolated from Curcuma longa root, has been commonly used treatment diseases. However, details how curcumin exerts neuroprotection remain uncertain. Here, we investigated protective effect on 1-methyl-4-phenylpyridinium ion-(MPP(+)-) stimulated primary astrocytes. Our results showed MPP(+) stimulation resulted significant production tumor necrosis factor (TNF)-α, interleukin (IL-6), and reactive oxygen species (ROS) mesencephalic Curcumin pretreatment decreased levels these cytokines while increased IL-10 expression MPP(+)-stimulated In addition, antioxidant glutathione (GSH) reduced ROS production. further TLR4 its downstream effectors NF-κB, IRF3, MyD88, TIRF are induced as well inhibited immunoreactivity morphological Together, data suggest might exert beneficial neuroinflammation