Optimization of a natural collagen scaffold to aid cell-matrix penetration for urologic tissue engineering.
作者： Yan Liu , Shantaram Bharadwaj , Sang J. Lee , Anthony Atala , Yuanyuan Zhang
DOI: 10.1016/J.BIOMATERIALS.2009.04.008
关键词: Cell 、 Cell growth 、 Scaffold 、 Materials science 、 Submucosa 、 Tissue engineering 、 Biomedical engineering 、 In vivo 、 Decellularization 、 Extracellular matrix
摘要: Abstract The goal of this study was to fabricate a 3-dimensional (3-D) porous scaffold derived from bladder submucosa (BSM) and further recellularize the with human cells for cell-based urethral tissue engineering. Fresh porcine BSM soaked peracetic acid (PAA) at different concentrations (0,1,3,5 10%) then treated Triton X-100 decellularization. DNA content analysis showed that nuclear material removed scaffold. Treatment 5% PAA led high porosity on surface matrix retention less cellular maintained about 75% normal tensile strength. In 3-D dynamic culture, formed even multiple layers matrix. Cells also penetrated deeper into lamina propria compared untreated Immunocytochemical staining indicated grafted expressed urothelial- smooth muscle-specific markers both, in vitro vivo. This demonstrates decellularized/oxidized possesses cell infiltration Further, seeded grown significantly promoted cell–matrix penetration growth Scaffolds such characteristics have potential applications urological
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