The Proliferation in Suspension of the Progenitors of the Blast Cells in Acute Myeloblastic Leukemia

摘要: A minority of blast cells in acute myeloblastic leukemia (AML) form colonies culture methylcellulose when stimulated by media conditioned normal leukocytes the presence phytohemagglutinin (PHA-LCM). Blast can be replated successfully, either as pooled or suspensions from single colonies. However, plating efficiency declines with repeated passages, and more than four subcultures have not been achieved. In this study, populations were cultured suspension, fetal calf serum, alpha-minimal essential medium PHA-LCM. 17 18 patients, exponential growth clonogenic was maintained for six to seven days without reculturing. Colonies obtained progenitors taken liquid obtain secondary (PE2). 14 cases, value increased. three instances, PE2 fell following methylcellulose. When suspension recultured, continued. nine 70 days. During time, maintained. Results experiments using velocity sedimentation separation analysis consistent view that increase a manifestation their self-renewal capacity. The observations also support model progenitor contains postulate these are capable only but determination-like events leading loss proliferative