The sensitivity to cytosine arabinoside of the blast progenitors of acute myeloblastic leukemia.

摘要: Two culture methods are available for the study of blast cells acute myeloblastic leukemia (AML). One is an assay clonogenic precursors; it depends on their ability to form colonies in presence methylcellulose and suitable growth factors. The other assesses suspension culture, where measured by increasing numbers cells. We have compared two as assays cytotoxic effects chemotherapeutic drug cytosine arabinoside (Ara-C). Marked patient- to-patient variation was found using either method; however, slopes dose-response curves were usually greater when exposed rather than methylcellulose. Control experiments showed that difference could not be explained carry-over from cultures plates suspensions assessed. Further, survival Adriamycin very similar, regardless which used. No correlation between D10 Ara-C values or However, a significant association with outcome response treatment regimen only agent such detected obtained same group 15 patients. Finally, feasibility experiment performed repeatedly during exponential over 238 days. A dose-related inhibition observed; no evidence seen emerging drug-resistant Nor did morphology change result exposure. conclude sensitivities AML dependent measurement methods, even techniques affecting cell proliferation compared. Measurements sensitivity may best interpreted bases systems understood.