Production and characteristics of an infusible oxygen-carrying fluid based on hemoglobin intramolecularly cross-linked with sebacic acid
作者： Enrico Bucci , Anna Razynska , Herman Kwansa , Barbara Matheson-Urbaitis , Michael O'hearne
DOI: 10.1016/S0022-2143(96)90006-2
关键词: Oncotic pressure 、 Red blood cell 、 Sebacic acid 、 Chemistry 、 Chromatography 、 Cooperativity 、 Oxygen 、 Reagent 、 Venous Plasma 、 Hemoglobin 、 Pathology and Forensic Medicine 、 General Medicine
摘要: Abstract Research on red cell substitutes requires the availability of oxygen-carrying fluids for physiologic experiments. This article describes procedure in-house production such a fluid. It contains hemoglobin-based oxygen carrier obtained by reacting human hemoglobin with bis-(3,5-dibromosalicyl) sebacate. reagent produces intramolecular cross-links between β82 lysines and α99 lysines, respectively. The half-saturation pressure (P50) fluid is near 34 mm Hg at 37°C, Hill's parameter n = 2.2. half-time intravascular retention 3.0 hours in rat 6.5 cat. Spectrophotometric analyses arterial venous plasma from an infused reveal efficient delivery to tissues carrier. Therefore, this new cross-linked can be produced quantities sufficient vivo evaluation affinity cooperativity adequate unloading plasma.
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