Determination of HER-2 status on FNAC material from breast carcinomas using in situ hybridization with dual chromogen visualization with silver enhancement (dual SISH).
作者： Elsa Beraki , Torill Sauer
关键词: In situ hybridization 、 First generation 、 Fine needle aspiration cytology 、 Polysomy 、 Medicine 、 Pathology 、 Dual color
摘要: During the last years, HER-2 status kits and protocols for chromogen visualization of hybridization signals have come on market. The first generation using used single color probes. second generation, now emerging market, uses dual visualization. aim this study has been to test a new kit (Ventana INFORM HER2 Dual Colour ISH Roche®) compare results with our in-house method(s). material consisted primarily cytological from invasive breast carcinomas in 49 women. SISH was done all histological specimens. specimens were treated according manufacturer’s recommendations. procedure modified several steps order adapt it material. Hybridization failed two showed concordant as amplified/not amplified. included cases had same expression situ components histology. Four IDC amplification (8.5%). Polysomy found cases. All except one concurred method(s) (1/47=2.1%). is suitable examination status. protocol must be optimized
cytojournal.com参考文章(17)
Yun Gong, William Sweet, Yi-Jing Duh, Larry Greenfield, Yuan Fang, Jianxin Zhao, Emily Tarco, W. Fraser Symmans, Jorma Isola, Nour Sneige, Chromogenic In Situ Hybridization Is a Reliable Method for DetectingHER2Gene Status in Breast Cancer American Journal of Clinical Pathology. ,vol. 131, pp. 490- 497 ,(2009) , 10.1309/AJCPI00TVGIGYXAA
Cheepsumon Suthipintawong, Anthony S-Y Leong, Keeng-Wai Chan, Songkhun Vinyuvat, Immunostaining of estrogen receptor, progesterone receptor, MIB1 antigen, andc-erbB-2 oncoprotein in cytologic specimens: A simplified method with formalin fixation Diagnostic Cytopathology. ,vol. 17, pp. 127- 133 ,(1997) , 10.1002/(SICI)1097-0339(199708)17:2<127::AID-DC8>3.0.CO;2-D
Jude A. Hudock, Cheryl A. Hanau, Randolph Christen, Marluce Bibbo, Expression of estrogen and progesterone receptors in cytologic specimens using various fixatives. Diagnostic Cytopathology. ,vol. 15, pp. 78- 83 ,(1996) , 10.1002/(SICI)1097-0339(199607)15:1<78::AID-DC17>3.0.CO;2-9
Wendy A. Raymond, Anthony S.-Y. Leong, An evaluation of potentially suitable fixatives for immunoperoxidase staining of estrogen receptors in imprints and frozen sections of breast carcinoma. Pathology. ,vol. 20, pp. 320- 325 ,(1988) , 10.3109/00313028809085211
Rita Nizzoli, Cecilia Bozzetti, Pellegrino Crafa, Nadia Naldi, Annamaria Guazzi, Beatrice Di Blasio, Roberta Camisa, Stefano Cascinu, Immunocytochemical evaluation of HER-2/neu on fine-needle aspirates from primary breast carcinomas. Diagnostic Cytopathology. ,vol. 28, pp. 142- 146 ,(2003) , 10.1002/DC.10257
V. Kuenen-Boumeester, A. M. Timmermans, E. M. C. A. de Bruijn, S. C. Henzen-Logmans, Immunocytochemical detection of prognostic markers in breast cancer; technical considerations. Cytopathology. ,vol. 10, pp. 308- 316 ,(1999) , 10.1046/J.1365-2303.1999.00192.X
Kevin C. Halling, Benjamin R. Kipp, Fluorescence in situ hybridization in diagnostic cytology Human Pathology. ,vol. 38, pp. 1137- 1144 ,(2007) , 10.1016/J.HUMPATH.2007.04.015
M Laakso, M Tanner, J Isola, Dual-colour chromogenic in situ hybridization for testing of HER-2 oncogene amplification in archival breast tumours. The Journal of Pathology. ,vol. 210, pp. 3- 9 ,(2006) , 10.1002/PATH.2022
G. Y. Kim, Y. L. Oh, Chromogenic in situ hybridization analysis of HER-2/neu status in cytological samples of breast carcinoma. Cytopathology. ,vol. 15, pp. 315- 320 ,(2004) , 10.1111/J.1365-2303.2004.00214.X
G. Kocjan, C. Bourgain, A. Fassina, B. Hagmar, A. Herbert, K. Kapila, I. Kardum-Skelin, V. Kloboves-Prevodnik, S. Krishnamurthy, H. Koutselini, B. Majak, W. Olszewski, B. Onal, Ž. Pohar-Marinšek, I. Shabalova, J. Smith, E. Tani, P. Vielh, H. Wiener, U. Schenck, F. Schmitt, The role of breast FNAC in diagnosis and clinical management: a survey of current practice Cytopathology. ,vol. 19, pp. 271- 278 ,(2008) , 10.1111/J.1365-2303.2008.00610.X