Biochemical and biophysical characterization of insulin granules isolated from rat pancreatic islets by an iso-osmotic gradient
作者： E.K. Matthews , D.B. McKay , M.D.L. O'Connor , J.L. Borowitz
DOI: 10.1016/0304-4165(82)90052-6
关键词: Biochemistry 、 Pancreatic islets 、 Granule (cell biology) 、 Centrifugation 、 Tonicity 、 Sucrose 、 Dynamic light scattering 、 Biophysics 、 Cytochrome c oxidase 、 Insulin 、 Biology
摘要: Abstract The size and polydispersity of insulin granules isolated from rat pancreatic islets by centrifugation on a linear iso-osmotic gradient (300 mosM) have been characterized quas-elastic light scattering photon correlation spectroscopy. separation the techniques was compared directly to isolation discontinuous gradients hypertonic sucrose (300–1950 greater efficiency assessed parallel measurements protein, insulin, cytochrome oxidase β-glucuronidase. Granules β-cells had mean particle diameter 342 nm, buoyant density 1.104, hydrated mass 23 femtograms maximal content 8–9.6·10 5 molecules pr granule.
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