Calcium exchangeability in subcellular fractions of pancreatic islet cells.
作者： J.L. Borowitz , E.K. Matthews
DOI: 10.1242/JCS.41.1.233
关键词:
摘要: The distribution and exchangeability of calcium within subcellular compartments rat pancreatic islet cells was determined in pulse-chase experiments following isotopic labelling with 45Ca2+. In unstimulated islets the mitochondrial fraction showed greatest, insulin secretory granule least, uptake 45Ca2+; subsequently rate loss from each compartment during 120 min order greater than microsomal fraction. On exposure to D-glucose, 16.8 mM, 45Ca all 3 increased after 5 30 stimulation. contrast, theophylline 10 mM caused a significant decrease content little effect on other compartments. These results are discussed relation importance disposition, exchangeability, mobility for initiation control secretion.
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