Human mid-trimester amniotic fluid stem cells cultured under embryonic stem cell conditions with valproic acid acquire pluripotent characteristics.

摘要: Human mid-trimester amniotic fluid stem cells (AFSC) have promising applications in regenerative medicine, being broadly multipotent with an intermediate phenotype between embryonic (ES) and mesenchymal (MSC). Despite this propluripotent phenotype, AFSC are usually cultured adherence a serum-based expansion medium, how conditions sustaining pluripotency might affect their remains unknown. We recently showed that early from first trimester fluid, which endogenously express Sox2 Klf4, can be reprogrammed to without viral vectors using the histone deacetylase inhibitor valproic acid (VPA). Here, we show under MSC contained subset of expressing telomerase, CD24, OCT4, C-MYC, SSEA4, but low/null levels SOX2, NANOG, KLF4, SSEA3, TRA-1–60, TRA-1–81, unable form embryoid bodies (EBs) or teratomas. In contrast, human ESC were smaller size, grew faster, formed colonies, upregulated OCT4 expressed KLF4 not TRA-1–81. Supplementation VPA for 5 days further induced expression now able EBs conclude AFSC, may isolated autologously during pregnancy ethics restriction, acquire pluripotent characteristics use ectopic factors. Our data suggest medium-dependant approach reflects true reprogramming selection prepluripotent cells.