Cell surface proteins in a cultured cell line of Drosophila melanogaster
作者： T.K. Johnson , L.A. Brown , R.E. Denell
DOI: 10.1016/0020-1790(84)90087-8
关键词: Metabolism 、 Glycoprotein 、 Trypsin 、 Cell culture 、 Biology 、 Glucosamine 、 Biochemistry 、 Lactoperoxidase 、 Drosophila melanogaster 、 Concanavalin A 、 Insect Science 、 Molecular biology
摘要: Abstract Cell surface proteins and glycoproteins of a cultured Drosophila melanogaster cell line were studied by two-dimensional electrophoresis. Proteins radiolabelled the lactoperoxidase method examined for hydrophilic or hydrophobic properties using phase separation technique, as well trypsin sensitivity. The populations labelled catalyzed radio-iodination compared to either metabolic incorporation tritiated glucosamine binding concanavalin A. general distribution characteristics are also discussed.
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