Variations in the levels of cyclic adenosine 3′:5′-monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3t:́5′-monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii

摘要: Abstract Particulate cell fractions of mycelium Mucor rouxii contain adenylate cyclase activity which can be partially solubilized by 2% Lubrol PX. The enzyme requires Mn 2+ and its is not modified NaF or guanosine nucleotides. Mycelial extracts also cyclic adenosine 3′:5′-monophosphate phosphodiesterase activity, 60% soluble. This shows characteristic low K m (1 μ ) for AMP does hydrolyze 3′:5′-monophosphate. It ions maximal inhibited methylxanthines activated imidazole. Both enzymatic activities vary during the aerobic life cycle fungus. spores have highest levels cAMP phosphodiesterase, decrease development. At round stage, reaches 40% varies only slightly thereafter. this stage specific 25% ungerminated spores, from on, increases up to end logarithmic phase. Intracellular been measured germination. variations intracellular level are tentatively explained unequal in phosphodiesterase. A continuous increase extracellular development has found, cannot accounted solely diesterase activities.