Tissue Content of Hydroxyestrogens in Relation to Survival of Breast Cancer Patients

摘要: Purpose: The main goal of our study was to assess estrogen contents breast tumor tissues, having different receptor status, in relation long-term follow-up patients. Experimental Design: Twenty-one cancer cases, all collected from January 1986 1988 at the M. Ascoli Cancer Hospital Centre Palermo, were included and compared with 6 healthy women as a control group. Average time patients 144 ± 10 months. status tissues determined by both ligand binding immunohistochemical assays. A high performance liquid chromatography-based approach, jointly gas chromatography/mass spectrometry, used identify measure estrogens, various hydroxyestrogens, their methoxy derivatives normal tissues. Results: Although variable concentrations hydroxylated estrogens detected, they consistently accounted for >80% estrogens. Significantly greater amounts 2- 4-hydroxyestradiol, along marked increase 16α-hydroxyestrone (OHE1), observed respect tissues. significant positive association elevated 16αOHE1 ( P = 0.015) alive, leading significantly lower 0.043) 2OHE1:16αOHE1 ratio values. Conversely, values 4:2 hydroxy+methoxy 0.006) deceased patients. Using cutoff 1.2 150 fmol/mg tissue we achieved clear-cut separation patients, over-cutoff 147 months under showing only 47 median survival 0.00008). Conclusions: Our data imply that individual hydroxyestrogens may have distinct role onset clinical progression cancer, levels being turn associated prolonged