Modulation of cell differentiation in bone tissue engineering constructs cultured in a bioreactor.

摘要: In summary, many factors can influence the osteoblastic differentiation of marrow stromal cells when cultivated on three-dimensional tissue engineering scaffolds. creating ideal bone constructs consisting a combination scaffold, cells, and bioactive factors; flow perfusion bioreactor is much more suitable culture environment than static in well plates. The eliminates mass transport limitations to scaffold interior provides mechanical stimulation seeded through fluid shear. Scaffold properties such as pore size impact cell differentiation, especially culture. addition, bone-like extracellular matrix created by vitro porous scaffolds creates an osteoinductive for other populations. Therefore, have excellent potential regeneration applications clinic. However, work required optimize this strategy. A biodegradable material with integrity similar native degradation rate formation would be material. It also yet unclear what optimum amount generated are maximize formation. Finally, better characterization patterns within needed understand relationship between shear creation scaffold/culture combination.