Behaviour of multipotent maxillary bone-derived cells on beta-tricalcium phosphate and highly porous bovine bone mineral.
作者： Michael Payer , Birgit Lohberger , Elke Stadelmeyer , Christina Bartmann , Reinhard Windhager
DOI: 10.1111/J.1600-0501.2009.01856.X
关键词:
摘要: Objectives: The aim of this study was to test the applicability multipotent maxillary cells (MMC) for cell therapy concepts and evaluate their in vitro behaviour on two different bone substitutes. Material methods: Cells isolated from 10 donors were expanded using media containing human platelet lysate (HPL) replacing foetal bovine serum differentiated towards both osteogenic adipogenic lineage. Surface markers determined by fluorescence-activated sorting analysis. Calcium deposits, alkaline phosphatase (ALP) osteocalcin (OC) used as biomarkers differentiation. Oil Red O verify differentiation. The lineage undifferentiated controls further cultured natural mineral origin (BioOss®) β-tricalcium phosphate (Vitoss®) scaffolds. Scaffold efficacy migration evaluated with live imaging. Results: Isolated presented characteristics marrow (BM)-stromal could easily be clinical scales. expressed when inductive media. There no obvious differences growth between substitutes, but significantly higher OC expression observed BioOss® Both osteogenically lines ALP activity scaffolds. Conclusion: demonstrate comparable those BM-stromal cells. HPL can predictably clinical-scale expansion MMCs. grafting materials provide potential carrier loaded MMCs. To cite article: Payer M, Lohberger B, Stadelmeyer E, Bartmann C, Windhager R, Jakse N. Behaviour bone-derived highly porous mineral. Clin. Oral Impl. Res. 21, 2010; 699–708. doi: 10.1111/j.1600-0501.2009.01856.x
sci-hub.se 参考文章(63)
James Ra, Boyne Pj, Grafting of the maxillary sinus floor with autogenous marrow and bone. Journal of oral surgery. ,vol. 38, pp. 613- 616 ,(1980)
Hideharu Hibi, Shunsuke Baba, Yoichi Yamada, Minoru Ueda, A novel approach to periodontal tissue regeneration with mesenchymal stem cells and platelet-rich plasma using tissue engineering technology: A clinical case report. International Journal of Periodontics & Restorative Dentistry. ,vol. 26, pp. 363- 369 ,(2006)
Richard M. OʼKeeffe, Barry L. Riemer, Spencer L. Butterfield, Harvesting of Autogenous Cancellous Bone Graft from the: Proximal Tibial Metaphysis A Review of 230 Cases Journal of Orthopaedic Trauma. ,vol. 5, pp. 469- 474 ,(1991) , 10.1097/00005131-199112000-00014
Heidi L. Holtorf, John A. Jansen, Antonios G. Mikos, Modulation of cell differentiation in bone tissue engineering constructs cultured in a bioreactor. Advances in Experimental Medicine and Biology. ,vol. 585, pp. 225- 241 ,(2006) , 10.1007/978-0-387-34133-0_16
George F. Muschler, Cynthia Boehm, Kirk Easley, Aspiration to Obtain Osteoblast Progenitor Cells from Human Bone Marrow: The Influence of Aspiration Volume* Journal of Bone and Joint Surgery, American Volume. ,vol. 79, pp. 1699- 1709 ,(1997) , 10.2106/00004623-199711000-00012
Sunday O. Akintoye, Thanh Lam, Songtao Shi, Jaime Brahim, Michael T. Collins, Pamela G. Robey, Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals Bone. ,vol. 38, pp. 758- 768 ,(2006) , 10.1016/J.BONE.2005.10.027
Harutsugi Abukawa, Hidetomi Terai, Didier Hannouche, Joseph P. Vacanti, Leonard B. Kaban, Maria J. Troulis, Formation of a mandibular condyle in vitro by tissue engineering Journal of Oral and Maxillofacial Surgery. ,vol. 61, pp. 94- 100 ,(2003) , 10.1053/JOMS.2003.50015
Manuela E. Gomes, Heidi L. Holtorf, Rui L. Reis, Antonios G. Mikos, Influence of the porosity of starch-based fiber mesh scaffolds on the proliferation and osteogenic differentiation of bone marrow stromal cells cultured in a flow perfusion bioreactor. Tissue Engineering. ,vol. 12, pp. 801- 809 ,(2006) , 10.1089/TEN.2006.12.801
B. D. Boyan, R. Batzer, K. Kieswetter, Y. Liu, D. L. Cochran, S. Szmuckler-Moncler, D. D. Dean, Z. Schwartz, Titanium surface roughness alters responsiveness of MG63 osteoblast‐like cells to 1α,25‐(OH)2D3 Journal of Biomedical Materials Research. ,vol. 39, pp. 77- 85 ,(1998) , 10.1002/(SICI)1097-4636(199801)39:1<77::AID-JBM10>3.0.CO;2-L
Michaela Endres, DW Hutmacher, AJ Salgado, Christian Kaps, J Ringe, RL Reis, Michael Sittinger, Arthur Brandwood, Jan-Thorsten Schantz, None, Osteogenic Induction of Human Bone Marrow-Derived Mesenchymal Progenitor Cells in Novel Synthetic Polymer–Hydrogel Matrices Tissue Engineering. ,vol. 9, pp. 689- 702 ,(2003) , 10.1089/107632703768247386