Pre-osteoblast infiltration and differentiation in highly porous apatite-coated PLLA electrospun scaffolds

作者: Bryce M. Whited , Jon R. Whitney , Matthias C. Hofmann , Yong Xu , Marissa N. Rylander

DOI: 10.1016/J.BIOMATERIALS.2010.12.003

关键词: BiophysicsMineralization (biology)Tissue engineeringMaterials scienceScaffoldElectrospinningCell migrationOsteoblastBiomedical engineeringAlkaline phosphataseApatite

摘要: Electrospun polymer/apatite composite scaffolds are promising candidates as functional bone substitutes because of their ability to allow pre-osteoblast attachment, proliferation, and differentiation. However these structures usually lack an adequate pore size permit sufficient cell migration colonization the scaffold. To overcome this limitation, we developed apatite-coated electrospun PLLA scaffold with varying porosity by utilizing a three-step water-soluble PEO fiber inclusion, dissolution, mineralization process. The temporal spatial dynamics into were quantified determine effects enhanced on infiltration. MC3T3-E1 was found be function both initial content time. Scaffolds greater (50% 75% PEO) had drastically accelerated infiltration in addition distribution throughout when compared lower (0% 25% PEO). Furthermore, apatite substrate significantly upregulated alkaline phosphatase activity, osteocalcin content, cell-mediated alone. These findings suggest that such enhances infiltration, colonization, maturation vitro may lead overall improved formation implanted vivo.

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