Polymerase chain reaction assay identifies North American members of the Culex pipiens complex based on nucleotide sequence differences in the acetylcholinesterase gene Ace.2.

作者： Harry M. Savage , Stephen Aspen

DOI:

关键词: Culex pipiens 、 Intron 、 Genetics 、 Biology 、 Parasite hosting 、 Arbovirus 、 Nucleic acid sequence 、 Culex quinquefasciatus 、 Gene 、 Polymerase chain reaction

摘要: Nucleotide sequence differences in the acetylcholinesterase gene Ace.2 were used to develop an assay distinguish among North American mosquitoes of Culex pipiens complex. Taxon-specific polymerase chain reaction primers based on within intron 2 sibling species Cx. Linneaus and quinquefasciatus Say their F 1 hybrids. This may be confirm composition mosquito pools, identify individual specimens collected arbovirus surveillance programs other studies, define zones hybridization.

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Polymerase chain reaction assay identifies North American members of the Culex pipiens complex based on nucleotide sequence differences in the acetylcholinesterase gene Ace.2.

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Polymerase chain reaction assay identifies North American members of the Culex pipiens complex based on nucleotide sequence differences in the acetylcholinesterase gene Ace.2.

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