Host factors that affect Ty3 retrotransposition in Saccharomyces cerevisiae

摘要: The retrovirus-like element Ty3 of Saccharomyces cerevisiae integrates at the transcription initiation region RNA polymerase III. To identify host genes that affect transposition, a collection insertion mutants was screened using genetic assay in which activates expression tRNA suppressor. Fifty-three loci were identified this screen. Corresponding knockout tested for ability to mobilize galactose-inducible Ty3, marked with HIS3 gene. Of 42 tested, 22 had phenotypes similar those displayed original assay. proteins encoded by defective are involved chromatin dynamics, transcription, processing, protein modification, cell cycle regulation, nuclear import, and unknown functions. These induced assayed Gag3p protein, integrase, cDNA, integration upstream chromosomal tDNAVal(AAC) genes. Most differences from wild type one or more intermediates, although these typically not as severe defect. Because relatively large number affecting retrotransposition can be yeast because majority have mammalian homologs, approach provides an avenue identification potential antiviral targets.