Ty3 requires yeast La homologous protein for wild-type frequencies of transposition.
作者： Michael Aye , Suzanne B. Sandmeyer
DOI: 10.1046/J.1365-2958.2003.03568.X
关键词:
摘要: The Saccharomyces cerevisiae retrovirus-like element Ty3 inserts specifically into the initiation sites of genes transcribed by RNA polymerase III (pol III). A strain with a disruption LHP1, which encodes homologue autoantigen La protein, was recovered in screen for mutants defective transposition. Transposition target composed divergent tRNA decreased eightfold. In lhp1 mutants, polyproteins were produced at wild-type levels, assembled virus-like particles (VLPs) and processed efficiently. amount cDNA associated these about half control early times, but approached level after 48 h induction. integration examined two genomic gene families plasmid-borne promoters. Integration significantly one families, only slightly second family. These findings suggest that Lhp1p contributes to synthesis, might also act target-specific step, such as integration.
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