Accumulation of recessive lethal mutations in Saccharomyces cerevisiae mlh1 mismatch repair mutants is not associated with gross chromosomal rearrangements.
作者： Julie Akiko Heck , David Gresham , David Botstein , Eric Alani
DOI: 10.1534/GENETICS.106.059311
关键词: DNA mismatch repair 、 Genetics 、 Biology 、 Gene 、 Chromosome instability 、 MLH1 、 Gene rearrangement 、 Mutation 、 Molecular biology 、 Frameshift mutation 、 Saccharomyces cerevisiae
摘要: We examined mismatch repair (MMR)-defective diploid strains of budding yeast grown for ∼160 generations to determine whether decreases in spore viability due the uncovering recessive lethal mutations correlated with an increase gross chromosomal rearrangements (GCRs). No GCRs were detected despite dramatic viability, suggesting that frameshift and/or other unrepaired DNA replication lesions play a greater role than instability decreasing MMR-defective strains.
genetics.org
princeton.edu参考文章(24)
Kyungjae Myung, Abhijit Datta, Clark Chen, Richard D. Kolodner, SGS1, the Saccharomyces cerevisiae homologue of BLM and WRN, suppresses genome instability and homeologous recombination. Nature Genetics. ,vol. 27, pp. 113- 116 ,(2001) , 10.1038/83673
T A Prolla, D M Christie, R M Liskay, Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene. Molecular and Cellular Biology. ,vol. 14, pp. 407- 415 ,(1994) , 10.1128/MCB.14.1.407
R. D. Kolodner, R. A. G. Reenan, Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions. Genetics. ,vol. 132, pp. 975- 985 ,(1992) , 10.1093/GENETICS/132.4.975
Marsha S Williamson, John C Game, Seymour Fogel, None, MEIOTIC GENE CONVERSION MUTANTS IN SACCHAROMYCES CEREVISIAE. I. ISOLATION AND CHARACTERIZATION OF pms1-1 AND pms1-2 Genetics. ,vol. 110, pp. 609- 646 ,(1985) , 10.1093/GENETICS/110.4.609
Francene J. Lemoine, Natasha P. Degtyareva, Kirill Lobachev, Thomas D. Petes, Chromosomal Translocations in Yeast Induced by Low Levels of DNA Polymerase: A Model for Chromosome Fragile Sites Cell. ,vol. 120, pp. 587- 598 ,(2005) , 10.1016/J.CELL.2004.12.039
Gerald T. Marsischky, Richard D. Kolodner, Biochemical characterization of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 complex and mispaired bases in DNA Journal of Biological Chemistry. ,vol. 274, pp. 26668- 26682 ,(1999) , 10.1074/JBC.274.38.26668
S. Chen, S. H. Bigner, P. Modrich, High rate of CAD gene amplification in human cells deficient in MLH1 or MSH6 Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 13802- 13807 ,(2001) , 10.1073/PNAS.241508098
J. A. Heck, J. L. Argueso, Z. Gemici, R. G. Reeves, A. Bernard, C. F. Aquadro, E. Alani, Negative epistasis between natural variants of the Saccharomyces cerevisiae MLH1 and PMS1 genes results in a defect in mismatch repair Proceedings of the National Academy of Sciences of the United States of America. ,vol. 103, pp. 3256- 3261 ,(2006) , 10.1073/PNAS.0510998103
M. C. Earley, G. F. Crouse, The role of mismatch repair in the prevention of base pair mutations in Saccharomyces cerevisiae Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 15487- 15491 ,(1998) , 10.1073/PNAS.95.26.15487
J. D. Hawk, L. Stefanovic, J. C. Boyer, T. D. Petes, R. A. Farber, Variation in efficiency of DNA mismatch repair at different sites in the yeast genome. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 102, pp. 8639- 8643 ,(2005) , 10.1073/PNAS.0503415102