Induction of rat hepatic P4501A1 by organic extracts from airborne particulate matter in Santiago, Chile.
作者： L. Quiñones , L. Gil
DOI: 10.3109/00498259509061835
关键词: Composition (visual arts) 、 Stereochemistry 、 Enzyme inducer 、 Particulates 、 Carcinogen 、 Chemistry 、 Microsome 、 Polyclonal antibodies 、 Isozyme 、 Chromatography 、 Cytochrome P450
摘要: 1. Organic extracts from particulate matter collected in downtown Santiago, Chile, 1990 were administered to female Wistar rats.2. Extracts shifted the maximal absorption wavelength P450 spectra of reduced-CO complex hepatic microsomal 450 448 nm, and enhanced total content P450. In addition, substantial increases aryl hydrocarbon hydroxylase, ethoxyre-sofin O-deethylase ethoxycoumarin activities observed, whereas aminopyrine N-demethylase activity was not affected by treatment.3. Western blotting using polyclonal antibodies against P4501A isozymes showed appearance a distinct intense P4501A1 band microsomes rat pretreated with air particle extracts, observed control rat. On other hand, intensity P4501A2 isoenzyme apparently affected.4. These findings suggest that organic airborne modify composition liver inducing t...
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