GSK3β inhibits epithelial-mesenchymal transition via the Wnt/β-catenin and PI3K/Akt pathways.
作者：
关键词: Epithelial–mesenchymal transition 、 Cancer research 、 Wortmannin 、 Protein kinase B 、 Medicine 、 PI3K/AKT/mTOR pathway 、 Catenin 、 Wnt signaling pathway 、 Proliferative vitreoretinopathy 、 GSK-3
摘要: Aim To investigate the regulatory mechanism of glycogen synthase kinase 3β (GSK3β) in epithelial-mesenchymal transition (EMT) process after proliferative vitreoretinopathy (PVR) induction. Methods Experimental PVR was induced by intravitreal injection retinal pigment epithelium (RPE) cells eyes rabbits. A PI3K/Akt inhibitor (wortmannin) and a GSK3β (LiCl) were also injected at different time during progress. Electroretinogram (ERG), ocular fundus photographs, B-scan ultrasonography used to observe Western blot test on extracted retina performed 1, 2, 4wk. The expression mesenchymal marker vimentin determined immunohistochemistry. Toxicity wortmannin LiCl evaluated ERG TdT-mediated dUTP nick-end labeling (TUNEL) assay. vitreous collected for metabolomic analysis. Results could significantly lead EMT, along with suppressed activation Wnt/β-catenin pathways. It verified that upregulating effectively inhibit EMT suppressing Conclusion inhibits via may be regarded as promising target experimental inhibition.
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