Identification of factors affecting technetium 99m leucocyte labelling by phagocytic engulfment and evelopment of an optimal technique

摘要: Human autologous leucocytes can be simply and reproducibly labelled by phagocytic engulfment of technetium 99m stannous colloid with high leucocyte-labelling efficiency (LLE), similar human biodistribution to indium-111-oxine good cell viability. A mean particle size 2.1 μms is optimal for phagocytosis the most important parameter in maintaining neutrophil uptake. It more critical than hitherto appreciated. When such variables as type colloid, purity starting materials, speed mixer rotation when preparing labelling leucocytes, heparin concentration, freshness preparation, sterilising membrane filter used incubation time cells are rigidly controlled, consistent efficiencies excess 90% obtained predominance. The lyophilised kit tested produced suboptimal results.