General Introduction to Cytochemical Bioassays
作者： J. Chayen , Lucille Bitensky
DOI: 10.1016/B978-0-407-00344-6.50006-2
关键词: Cytochemistry 、 Bioassay 、 Cytochemical bioassay 、 Biochemistry 、 Biology 、 Cell biology 、 Reaction product 、 Chemical activity
摘要: Publisher Summary This chapter focuses on quantitative chemistry, used in cytochemical bioassays. The methods of cytochemistry depend chilling the tissue, and sectioning it at low temperature, without producing any observable ice artifact. aim is to precipitate colored reaction product cell which chemical activity resides. greatest advantage bioassay system its use defining mode action hormones. approach allows measurement metabolic changes solely target cells. consequences attendant exposure cells hormone can be measured sequentially. flexibility makes ideal for studying interaction among possible agonists; effects other components plasma; investigating presence antibodies or agents against receptor.
sci-hub.st 参考文章(14)
I.M Holdaway, LesleyH Rees, J Landon, Circulating corticotrophin levels in severe hypopituitarism and in the neonate. The Lancet. ,vol. 302, pp. 1170- 1172 ,(1973) , 10.1016/S0140-6736(73)92938-3
J. CHAYEN, N. LOVERIDGE, J. R. DALY, A sensitive bioassay for adrenocorticotrophic hormone in human plasma. Clinical Endocrinology. ,vol. 1, pp. 219- 233 ,(1972) , 10.1111/J.1365-2265.1972.TB00393.X
J. CHAYEN, J.R. DALY, N. LOVERIDGE, LUCILLE BITENSKY, The cytochemical bioassay of hormones. Recent Progress in Hormone Research. ,vol. 32, pp. 33- 72 ,(1976) , 10.1016/B978-0-12-571132-6.50009-9
J. Chayen, Lucille Bitensky, Metabolism of Rheumatoid and Non-Rheumatoid Synovial Lining Cells Karger Publishers. pp. 59- 74 ,(1982) , 10.1159/000406520
J. Chayen, N. Loveridge, J. R. Daly, The measurable effect of low concentrations (pg-ml) of ACTH on reducing groups of adrenal cortex maintained in organ culture. Clinical Science. ,vol. 41, ,(1971) , 10.1042/CS041002PB
J. Chayen, The Cytochemical Approach to Hormone Assay International Review of Cytology-a Survey of Cell Biology. ,vol. 53, pp. 333- 396 ,(1978) , 10.1016/S0074-7696(08)62245-7
O TROWELL, The culture of mature organs in a synthetic medium Experimental Cell Research. ,vol. 16, pp. 118- 147 ,(1959) , 10.1016/0014-4827(59)90201-0
H. Muir, I. Olsen, M. F. Dean, G. Harris, Direct transfer of a lysosomal enzyme from lymphoid cells to deficient fibroblasts Nature. ,vol. 291, pp. 244- 247 ,(1981) , 10.1038/291244A0
Maria L. Dufau, Carole R. Mendelson, Kevin J. Catt, A highly sensitive in vitro bioassay for luteinizing hormone and chorionic gonadotropin: testosterone production by dispersed Leydig cells. The Journal of Clinical Endocrinology and Metabolism. ,vol. 39, pp. 610- 613 ,(1974) , 10.1210/JCEM-39-3-610
MICHAEL G. BESSER, DAVID N. ORTH, WENDELL E. NICHOLSON, RICHARD L. BYYNY, KAORU ABE, JEAN P. WOODHAM, Dissociation of the Disappearance of Bioactive and Radioimmunoreactive ACTH from Plasma in Man The Journal of Clinical Endocrinology and Metabolism. ,vol. 32, pp. 595- 603 ,(1971) , 10.1210/JCEM-32-5-595