Biosensors, Foodborne Pathogen Detection
作者： Arun K. Bhunia , Viswaprakash Nanduri , Euiwon Bae , E. Dan Hirleman
DOI: 10.1002/9780470054581.EIB158
关键词: Nanotechnology 、 Fiber optic sensor 、 Pathogen 、 Foodborne pathogen 、 Biochip 、 Surface plasmon resonance 、 Aptamer 、 Biosensor 、 Pathogen detection 、 Biology
摘要: In recent years, foodborne pathogens have become a significant concern due to increasing numbers of outbreaks and the resulting fatalities economic losses. addition improving hygienic practices in food production preparation, improvement pathogen testing is urgently needed. Conventional culture-based methods are lengthy, involve multiple steps, lack sensitivity. Even so-called antibody- or nucleic acid-based “rapid methods” require minimum 24–48 h complete. Faster more sensitive detection needed, biosensor-based capacity meet that need. this review, we describe developments area select sensors show promise for detection, including Fourier Transform InfraRed spectroscopy (FTIR), light scattering, surface plasmon resonance (SPR), fiber optic, microfluidic protein biochip, mammalian cell-based sensors. FTIR light-scattering considered label-free since they do not any probes labeling reagents. SPR semi-label-free method it requires recognition molecules such as antibodies, acids, aptamers, bacteriophages detection. The remaining reagents probes. Application utilization these biosensors also highlighted. Keywords: foodborne pathogen; biosensor; FTIR; fiber optic sensor; light-scattering sensor; biochip; cell-based biosensor
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