Genetic Diversity of Transcriptional Activator-Like Effector Genes in Chinese Isolates of Xanthomonas oryzae pv. oryzicola

摘要: Xanthomonas oryzae pv. oryzicola causes bacterial leaf streak (BLS), a devastating disease of rice in Asia countries. X. utilizes repertoires transcriptional activator-like effectors (TALEs) to manipulate host resistance or susceptibility; thus, TALEs can determine the outcome BLS. In this report, we studied genetic diversity putative tale genes 65 strains that originated from nine provinces southern China. Genomic DNAs were digested with BamHI and hybridized an internal fragment avrXa3, gene originating related pathogen, oryzae, which blight (BLB). Southern blot analysis indicated contained variable number (9 22) avrXa3-hybridizing fragments (e.g., genes). Based on size hybridizing bands, classified into 14 genotypes (designated 1 14), 3 10 represented 29.23 24.64% total, respectively. A high molecular weight (HMWB; ≈6.0 kb) was present 12 genotypes, sequence HMWB revealed presence C-terminally truncated tale, insertion element IS1403, encoding phosphoglycerate mutase endonuclease V. Primers developed 6.0-kb showed potential genotyping by polymerase chain reaction. Virulence assessed 23 cultivars containing different for BLB. The could be grouped pathotypes (I XIV), grouping almost identical categories determined genotypic analysis. general, higher numbers more virulent than fewer tales. results also indicate there are no gene-for-gene relationships between tested lines strains. To our knowledge, is first description based