Apolipoprotein B synthesis in humans: liver synthesizes only apolipoprotein B-100.
作者： Stephen B. Edge , Jeffrey M. Hoeg , Philip D. Schneider , H.Bryan Brewer
DOI: 10.1016/0026-0495(85)90022-8
关键词: Gel electrophoresis 、 Internal medicine 、 Polyacrylamide gel electrophoresis 、 Apolipoprotein B 、 Centrifugation 、 Isotopes of sulfur 、 Endocrinology 、 Very low-density lipoprotein 、 Chylomicron 、 Organ culture 、 Biology
摘要: Apolipoprotein (apo) B-100 and B-48 are prominent apolipoproteins in VLDL, IDL, chylomicrons. Organ cultures of normal adult human liver were established to ascertain the form apo B synthesized by hepatocytes humans. Human was minced incubated 15 mL methionine-free RPMI-1640 medium with 10% dialyzed fetal calf serum plus 250 microCi 35S-methionine for eight hours at 37 degrees C. Lipoproteins secreted isolated ultracentrifugation content newly determined quantitation radioactivity apoB-100 apoB-48 bands after separation 3% NaDodSO4 gel electrophoresis. In eight-hour period, 2.5% 3.2% added TCA-precipitable protein which 0.34% B. Ninety-nine percent LDL electrophoretic band. No significant detected Eighty-nine total VLDL 8% IDL LDL, respectively. These results establish that organ culture synthesizes but not B-48.
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