Photo‐induced protein cross‐linking to 5S RNA and 28–5.8S RNA within rat‐liver 60S ribosomal subunits
作者： Anne PALEOLOGUE , Anne-Marie REBOUD , Jean-Paul REBOUD
DOI: 10.1111/J.1432-1033.1985.TB08956.X
关键词: Molecular biology 、 Ribosome 、 Biology 、 Non-coding RNA 、 RNA polymerase I 、 RNA 、 5S ribosomal RNA 、 Biochemistry 、 RNA editing 、 Ribosomal RNA 、 5.8S ribosomal RNA
摘要: Rat liver 60S ribosomal subunits were irradiated with 254-nm ultraviolet light (1.26 X 10(4) quanta/subunit), under conditions which preserved their functional activity. Cross-linked RNA-protein complexes recovered after unreacted proteins had been removed by repeated acetic acid extractions. Proteins linked to the whole rRNA, 5S RNA and 28-5.8 S RNAs identified two-dimensional gel electrophoresis hydrolysis ribonucleases T1 A. Our results showed that numerous interact rRNAs (at least ten RNA, eight among these three are common both) have discussed in of all available data.
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