Platelet glycoprotein (Gp) IX associates with GpIb alpha in the platelet membrane GpIb complex.
作者： G Wu , FJ Meloni , SS Shapiro
DOI: 10.1182/BLOOD.V87.7.2782.BLOODJOURNAL8772782
关键词: Monoclonal antibody 、 Biochemistry 、 Alpha (ethology) 、 Glycoprotein 、 Platelet 、 Platelet membrane glycoprotein 、 Peptide 、 Polyclonal antibodies 、 Antibody 、 Chemistry
摘要: The platelet membrane glycoprotein (Gp) Ib complex consists of four polypeptides: the disulfide-linked GpIb alpha and beta subunits; GpIX, tightly, but noncovalently associated with alpha-beta; more weakly GpV. It is not certain whether association GpIX to Gplb alpha-beta via GpIB alpha, beta, or both subunits, although recently published evidence implicates an interaction beta. We have investigated using polyclonal rabbit antibodies raised by immunization purified glycocalicin synthetic peptide sequences from respectively, as well monoclonal directed against (FMC-25) (AP-1). performed two types experiments, either platelets. (1) When wells were coated nonreduced complex, binding FMC-25 was inhibited 73% antibody, only 30% antibody; when reduced same 86% 13%, respectively. then incubated captured reactivity. reacted strongly; in contrast, reactivity retained complex. In reciprocal experiment, AP-1- bound radiolabeled FMC-25. (2) ability inhibit platelets studied ELISA flow cytometry. systems, significantly antibody. conclude that strongly membrane.
ashpublications.org
bloodjournal.org参考文章(17)
X Du, L Beutler, C Ruan, PA Castaldi, MC Berndt, Glycoprotein Ib and glycoprotein IX are fully complexed in the intact platelet membrane. Blood. ,vol. 69, pp. 1524- 1527 ,(1987) , 10.1182/BLOOD.V69.5.1524.1524
Sheila Timmons, Jacek Hawiger, Isolation of human platelets by albumin gradient and gel filtration. Methods in Enzymology. ,vol. 169, pp. 11- 21 ,(1989) , 10.1016/0076-6879(89)69046-5
P W Modderman, L G Admiraal, A Sonnenberg, A E von dem Borne, Glycoproteins V and Ib-IX form a noncovalent complex in the platelet membrane. Journal of Biological Chemistry. ,vol. 267, pp. 364- 369 ,(1992) , 10.1016/S0021-9258(18)48503-1
JA Lopez, B Leung, CC Reynolds, CQ Li, JE Fox, Efficient plasma membrane expression of a functional platelet glycoprotein Ib-IX complex requires the presence of its three subunits. Journal of Biological Chemistry. ,vol. 267, pp. 12851- 12859 ,(1992) , 10.1016/S0021-9258(18)42354-X
V Vicente, R A Houghten, Z M Ruggeri, Identification of a site in the alpha chain of platelet glycoprotein Ib that participates in von Willebrand factor binding. Journal of Biological Chemistry. ,vol. 265, pp. 274- 280 ,(1990) , 10.1016/S0021-9258(19)40226-3
Jose A Lopez, Stuart Weisman, David A Sanan, Tracey Sih, Margaret Chambers, Chester Q Li, Glycoprotein (GP) Ib beta is the critical subunit linking GP Ib alpha and GP IX in the GP Ib-IX complex. Analysis of partial complexes. Journal of Biological Chemistry. ,vol. 269, pp. 23716- 23721 ,(1994) , 10.1016/S0021-9258(17)31574-0
R R Montgomery, T J Kunicki, C Taves, D Pidard, M Corcoran, Diagnosis of Bernard-Soulier syndrome and Glanzmann's thrombasthenia with a monoclonal assay on whole blood. Journal of Clinical Investigation. ,vol. 71, pp. 385- 389 ,(1983) , 10.1172/JCI110780
Andreas N. WICKI, Kenneth J. CLEMETSON, The glycoprotein Ib complex of human blood platelets. FEBS Journal. ,vol. 163, pp. 43- 50 ,(1987) , 10.1111/J.1432-1033.1987.TB10734.X
Z M Ruggeri, L De Marco, L Gatti, R Bader, R R Montgomery, Platelets have more than one binding site for von Willebrand factor. Journal of Clinical Investigation. ,vol. 72, pp. 1- 12 ,(1983) , 10.1172/JCI110946
J. A. Lopez, D. W. Chung, K. Fujikawa, F. S. Hagen, E. W. Davie, G. J. Roth, The alpha and beta chains of human platelet glycoprotein Ib are both transmembrane proteins containing a leucine-rich amino acid sequence. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 2135- 2139 ,(1988) , 10.1073/PNAS.85.7.2135